Detection Method:Sandwich
Test principle:This assay employs a two-site sandwich ELISA to quantitate SDHD in samples. An antibody specific for SDHD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anySDHD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDHD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDHD bound in the initial step. The color development is stopped and the intensity of the color is measured.
Product Overview:SDHD, which stands for succinate dehydrogenase complex subunit D, is one of the two transmembrane subunits of the four-subunit succinate dehydrogenase protein complex that resides in the inner mitochondrial membrane. It also refers to the gene that codes for this protein.
Germline mutations in SDHD were first linked to hereditary paraganglioma in 2000. Since then, it has been shown that mutations in SDHB and to a lesser degree SDHC can cause paranglioma as well familial pheochromocytoma. Notably, the tumor spectrum is different for the different mutations. SDHB mutations often lead to metastatic disease that is extra-adrenal, while SDHD mutation related tumors are more typically benign, originating in the head and neck.
